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. 2016 Jul 12;100(6):1323–1334. doi: 10.1189/jlb.2A1114-534R

Figure 3. Change in expression of mRNAs encoding class 3 Semas during differentiation of monocytes into imDCs and mDCs.

Figure 3.

Total RNA was isolated from monocytes and monocyte-derived imDCs and mDCs and was analyzed for expression of genes encoding all class 3 Semas by SYBR Green semiquantitative real-time RT-PCR, as described in Materials and Methods. The fold change in each mRNA in imDCs and mDCs compared with monocytes is shown relative to the change in the expression of GAPDH RNA. The level of expression of each gene in monocytes was set to 1, as noted by the dotted, horizontal lines. Data represent the means ± se of samples run in triplicate and are representative of data from experiments using cells from 3 different donors, as seen in Table 2 (*P < 0.05; ***P < 0.001; ns, P > 0.05).