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. 2016 Jul 12;100(6):1323–1334. doi: 10.1189/jlb.2A1114-534R

Figure 6. Sema3A, -3C, and -3F promote mDC migration.

Figure 6.

Migration of human monocyte-derived mDCs after exposure to AP-Sema3A, AP-Sema3F, or AP control and to Sema3C-Fc or human IgG1 was evaluated using quantitative transwell chemotaxis kits without chemokine CCL19 (A) or in the presence of CCL19 (B), as described in Materials and Methods. (C) mDCs were preincubated with preimmune goat IgG (control IgG, black bars) or goat polyclonal anti-NRP-2 IgG (gray bars) before exposure to Semas and subsequent measurement of migration. All assays were performed in duplicate, and percent migration represents the percentage of input DCs added to the upper chamber that migrated into the lower chamber. Data are from 1 experiment and are representative of results from 5 different experiments for A and B and from 3 different experiments for C (*P < 0.05; **P < 0.01; ***P < 0.001; ns, P > 0.05).