TABLE 2.
Gene expression of class 3 Semas in human monocytes and DCs
| Donor | Gene | Relative amount of mRNA for each gene |
||
|---|---|---|---|---|
| Monocytes | imDCs | mDCs | ||
| 1 | GAPDH | (20.41) | (17.23) | (17.61) |
| Sema3A | (30.61) | 0.18 ± 0.01 (29.87) | 0.53 ± 0.06 (28.74) | |
| Sema3C | (28.61) | 12.68 ± 0.00 (21.77) | 25.4 ± 2.46 (21.15) | |
| Sema3F | (26.23) | 0.00 ± 0.00 (31.30) | 0.00 ± 0.00 (32.21) | |
| 2 | GAPDH | (19.80) | (18.00) | (18.09) |
| Sema3A | (31.39) | 1.71 ± 0.64 (28.92) | 3.07 ± 1.18 (28.17) | |
| Sema3C | (27.70) | 12.08 ± 4.04 (22.03) | 52.93 ± 7.83 (20.28) | |
| Sema3F | (26.42) | 0.01 ± 0.00 (31.24) | 0.01 ± 0.00 (31.88) | |
| 3 | GAPDH | (18.79) | (17.92) | (17.55) |
| Sema3A | (31.41) | 7.00 ± 2.61 (27.98) | 7.45 ± 2.23 (29.72) | |
| Sema3C | (27.73) | 23.46 ± 8.32 (22.54) | 67.30 ± 8.81 (23.13) | |
| Sema3F | (26.39) | 0.02 ± 0.00 (31.76) | 0.05 ± 0.25 (32.75) | |
Monocytes, monocyte-derived imDCs, and mDCs from 3 different donors were analyzed for class 3 Sema expression. The fold increase in level of mRNA encoding each Sema is indicated, with the PCR crossover threshold value for the amount of mRNA encoding each protein noted in parentheses. Fold increase in mRNA in imDCs and DCs is based on the level in monocytes and normalized to the change in GAPDH mRNA. No mRNA encoding Sema3B, -3D, -3E, or -3G was detected in all 3 donors.