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. 2016 Nov 14;213(12):2729–2744. doi: 10.1084/jem.20151920

Table 3. Reduction experiment eliminating one cytokine at a time from the cytokines added to the cells.

Factor reduction (−) CMS Non-CMS Sea level
SCF     0.2      0      0
TPO     31.1      0      0.1
Flt3     31.5      0      0
BMP4     0.1      0.1      0
VEGF     68.8      0      0
IL-3     2.3      0.1      0.6
IL-6     1.4      0.1      0.2
Epo     0.1      0      0.1

Relative proportion of CD235a marker after 3 wk under hypoxia eliminating one factor at a time. Eliminated cytokines are shown in column 1 for each experiment. The numbers under each population represent relative percentages of CD235a. The relative levels of CD235a in all populations with all factors in the media range in CMS (36–91.6%), non-CMS (0.2–1%), and sea level (3.1%–9.1%) are shown. Example: When TPO is eliminated in culture, 31.1% of cells (for CMS) were CD235a positive; for non-CMS and sea level, it was nil. Hence, CMS cells do not need TPO but sea-level and non-CMS cells do.