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. 2016 Nov 14;213(12):2553–2565. doi: 10.1084/jem.20160600

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© 2016 Wu et al.

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Figure 2. — Monocyte progeny are marked by Mafb-driven lineage tracing. (A) Macrophages (MΦ) in MafB-mCherry-Cre × R26-stop-YFP × Zbtb46-GFP mouse spleen, gated as in Fig. S1 A, are displayed for expression of Mafb-mCherry and lineage-tracing marker YFP in a two-color histogram, and classical DC subsets are compared for expression of YFP. Shown is one sample and a box plot representing first quartile, median, and third quartile frequencies (n ≥ 5 animals over at least three independent experiments). (B) Microglia in MafB-mCherry-Cre × R26-stop-YFP mouse brain, gated as in Fig. S1 B, are displayed for expression of Mafb-mCherry and YFP in a two-color histogram. Shown is one representative sample (n ≥ 3 animals over at least two independent experiments). (A and B) Numbers indicate percentage of cells within the indicated gate, and dotted gray lines show fluorescent signal measured in non-mCherry and non-YFP control samples. (C) Lineages in MafB-mCherry-Cre × R26-stop-YFP × Zbtb46-GFP mouse blood, gated as in Fig. S1 C, are compared for expression of YFP. Shown is a box plot representing first quartile, median, and third quartile frequencies (n ≥ 8 animals over at least four independent experiments). mo., monocytes. (D) Heat map showing relative gene expression in the indicated monocyte subsets for gene expression microarray probe sets differentially expressed in Ly-6C^hi and Ly-6C^lo monocytes. Shown are averages of two biological replicates, excluding one YFP⁻ Ly-6C^lo monocyte sample below quality control thresholds. (E) Monocytes identified in C, distinguished on the basis of Ly-6C expression (left), are compared for expression of YFP (right). Shown is one representative sample. (F) Maturing macrophages in MafB-mCherry-Cre × R26-stop-YFP × Zbtb46-GFP mouse small intestinal lamina propria, identified by surface markers as indicated (left) after pregating as in Fig. S1 D, are compared for expression of YFP (right). Shown is one representative sample (n = 4 animals over two independent experiments). (E and F) Numbers indicate percentage of cells within the indicated gate.