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. 2016 Nov 14;213(12):2553–2565. doi: 10.1084/jem.20160600

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© 2016 Wu et al.

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Figure 5. — Ly-6C⁺ monocyte-derived cells in the inflamed lung mature without expressing Zbtb46. (A) Experimental outline for intratracheal (i.t.) sensitization and challenge of MafB-mCherry-Cre × R26-stop-YFP × Zbtb46-GFP mice using HDM antigen. (B) Classical DCs and monocyte-derived cells in the lung, identified by surface markers as indicated after pregating as in Fig. S2 C, are compared for expression of Ly-6C, lineage-tracing marker YFP, and Zbtb46-GFP after treatment with vehicle (saline) or HDM antigen following the schedule outlined in A. Shown is one representative sample (n ≥ 4 HDM-treated animals over at least two independent experiments). Numbers indicate percentage of cells within the indicated gate. (C) Experimental outline for high-dose intratracheal sensitization and challenge of MafB-mCherry-Cre × R26-stop-YFP × Zbtb46-GFP mice using HDM antigen. (D and E) Classical DCs and monocyte-derived cells in the lung (D) or mediastinal LN (E), identified by surface markers as indicated, are compared for expression of YFP and Zbtb46-GFP after treatment with vehicle (saline) or HDM antigen following the schedule outlined in C. Shown is one representative sample (n = 2 HDM-treated animals over two independent experiments). Numbers indicate percentage of cells within the indicated gate.