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. 2016 Nov 14;213(12):2759–2772. doi: 10.1084/jem.20160612

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© 2016 Hu et al.

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Figure 4. — TMEM16F deficiency causes severe T cell exhaustion. (A–J) WT or TMEM16F-KO mice were i.v. infected with 4 × 10⁶ pfu LCMV clone 13 for 80 d. (A–D) Expression of PD-1 in GP33-tetramer–positive CD8 T cells (A and B) and coproduction of IFN-γ and TNF in CD8 T cells from spleen after restimulation with indicated epitopes (C and D) were analyzed by flow cytometry. n = 4 for WT and n = 3 for KO. (E–H) Expression of PD-1 in GP66-tetramer–positive CD4 T cells (E and F) and coproduction of IFN-γ and TNF in CD4 T cells from spleen after restimulation with GP66 (G and H) were analyzed by flow cytometry. n = 4 for WT; n = 3 for KO. (I and J) Expression of T-bet and Eomes in GP33-tetramer–positive CD8 T cells were analyzed by flow cytometry. Data are pooled from two experiments. n = 8 for WT; n = 6 for KO. MFI, mean fluorescence intensity. Results are displayed as mean ± SEM of two to five independent experiments. Student’s t test was used. *, P < 0.05; **, P < 0.01; ****, P < 0.0001.