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. 2016 Nov 14;213(12):2759–2772. doi: 10.1084/jem.20160612

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© 2016 Hu et al.

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Figure 5. — TMEM16F regulates T cell response in a cell-intrinsic manner. (A–C) WT or TMEM16F-deficient BM chimeric mice were i.v. infected with 4 × 10⁶ pfu LCMV clone 13 and sacrificed at day 150 after infection. Expression of PD-1 (A) and T-bet and Eomes (C) in GP33-tetramer–positive CD8 T cells, and cytokine production in CD8 T cells after stimulation with GP33 (B) were analyzed by flow cytometry. n = 4 for WT; n = 3 for KO. (D and E) Mixed BM chimera were infected as in A–C. Mice were sacrificed at day 150 after infection. Cytokine production in WT or KO CD8 T cells after stimulation with GP33 in D, and expression of T-bet and Eomes in GP33-tetramer–positive CD8 T cells in E, were determined by flow cytometry. n = 4 for each group. MFI, mean fluorescence intensity. Results are displayed as mean ± SEM of at least two independent experiments. *, P < 0.05; ***, P < 0.001; ****, P < 0.0001; ns, not significant, using Student’s t test.