Figure 6.

TMEM16F is required for control of chronic viral infection. (A–C) WT or TMEM16F-KO mice were i.v. infected with 4 × 10⁶ pfu LCMV clone 13 for 80 d (A and B) or 150 d (C). (A) Absolute number of GP33-tetramer–positive CD8 T cells per 10⁶ PBMCs was analyzed by flow cytometry at indicated time points after infection. n = 4 for WT; n = 3 for KO. (B and C) Virus loads in serum in B at indicated time points and in kidney at 138 d after infection in C were determined by focus assay. (B) n = 4 for WT n = 3 for KO; (C) n = 4. (D) WT or TMEM16F-deficient BM chimeric mice were i.v. infected with 4 × 10⁶ pfu LCMV clone 13 and sacrificed at day 150 after infection. Virus loads in kidney and liver of BM chimeric mice were determined by focus assay. LOD, limit of detection. n = 4 for WT; n = 3 for KO. Results are displayed as mean ± SEM of two to three independent experiments. Student’s t test was used. *, P < 0.05; **, P < 0.01; ****, P < 0.0001.