Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Nov 14;213(12):2759–2772. doi: 10.1084/jem.20160612

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2016 Hu et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

PMC Copyright notice

Figure 7. — TMEM16F is recruited to the synapse and requires microtubules for transport. (A–E) Jurkat cells expressing TMEM16F-RFP were co-cultured with Raji cells (A) or stimulated on coverslips coated with αCD3 (B–E). (A) Confocal microscopy analysis for localization of TMEM16F in Jurkat T cells co-cultured with control (unpulsed) or SEE-pulsed Raji B cells (SEE⁺). 2-µm z stack of images is shown. DIC, differential interference contrast. (B and C) Dynamics of TMEM16F at the TCR activation site were imaged by TIRF microscopy. Number of TMEM16F-positive spots was quantified in C. (D and E) Dynamics of TMEM16F at the TCR activation site were imaged by TIRF microscopy in Jurkat cells pretreated with vehicle (DMSO), 1 µM nocodazole, or 1 µM blebbistatin. (D) Number of TMEM16F-positive spots was quantified. Time zero is the start of recording. n = 5 for each group. (E) Trajectories of TMEM16F-positive spots were tracked by ImageJ. Bars, 5 µm. Data are representative of three independent experiments.