Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Nov 14;213(12):2745–2758. doi: 10.1084/jem.20160726

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2016 Nabekura and Lanier

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

PMC Copyright notice

Figure 2. — NK cells differentiate into memory NK cells and cytokine-activated NK cells after MCMV infection. NKp46-CreERT2 Tg mice with a heterozygous Rosa26-YFP allele (A) or homozygous Rosa26-YFP alleles (B–H) were treated with tamoxifen on days 0–4 and infected or not with MCMV on day 0. (A) The number of YFP⁺ NK cells and YFP⁻ NK cells in the blood over the course of infection. Data were pooled from two experiments (n = 7 in each group). *, P < 0.05; **, P < 0.01; ***, P < 0.005 versus Ly49H⁻ cells. (B) Expression of Ly49H and KLRG1 on YFP⁺ NK cells in the spleens of uninfected and infected mice on day 27 p.i. The percentages of Ly49H⁺KLRG1^high NK cells and Ly49H⁻KLRG1^high NK cells are shown. Expression of KLRG1 on YFP⁺ NK cells in the spleens of naive uninfected mice and on YFP⁺Ly49H⁺ memory NK cells and cytokine-activated YFP⁺Ly49H⁻ NK cells from MCMV-infected mice. Bold solid lines, bold dashed lines, thin solid lines, and thin dashed lines represent memory NK cells, cytokine-activated NK cells, naive Ly49H⁺ NK cells, and naive Ly49H⁻ NK cells, respectively. (C) Phenotype of YFP⁺ NK cells in the spleens of naive uninfected mice, and YFP⁺Ly49H⁺KLRG1^high memory NK cells and cytokine-activated YFP⁺Ly49H⁻KLRG1^high NK cells in the spleens of infected mice on day 25–32 p.i. (D) Expression of Granzyme B in YFP⁺ NK cells in the spleens of naive uninfected mice, and in memory YFP⁺Ly49H⁺KLRG1^high NK cells and cytokine-activated YFP⁺Ly49H⁻KLRG1^high NK cells in the spleens of infected mice on day 32 p.i. Bold solid lines, bold dashed lines, and thin lines represent memory NK cells, cytokine-activated NK cells, and naive NK cells, respectively. Filled histograms represent staining with an isotype-matched control Ig. Data are representative of more than five experiments (n = 2–6 in each experiment). (E) The percentages of YFP⁺ NK cells in naive uninfected mice on days 25–27 after tamoxifen treatment and the percentages of YFP⁺Ly49H⁺KLRG1^high memory NK cells and cytokine-activated YFP⁺Ly49H⁻KLRG1^high NK cells in the organs of tamoxifen-treated infected mice on days 25–27 p.i. *, P < 0.05; **, P < 0.01. (F) The percentages of YFP⁺Ly49H⁺ NK cells and YFP⁺Ly49H⁻ NK cells expressing a memory phenotype KLRG1^highLy6C⁺DNAM-1^low in the organs of tamoxifen-treated infected mice on days 25–27 p.i. Data are pooled from six experiments (n = 4–8 in each group). *, P < 0.01 versus memory cells. (G) Expression of CD49d on YFP⁺ NK cells in the blood of naive uninfected mice on day 25 after tamoxifen treatment and on YFP⁺Ly49H⁺KLRG1^high memory NK cells and cytokine-activated YFP⁺Ly49H⁻KLRG1^high NK cells in the blood of infected mice on day 25 p.i. Bold solid lines, bold dashed lines, and thin lines represent memory NK cells, cytokine-activated NK cells, and naive NK cells, respectively. A filled histogram represents staining with a control isotype-matched control Ig. Mean fluorescence intensity (MFI) of CD49d staining of NK cells are shown in H. Data are representative of two experiments (n = 4–6 in each group). *, P < 0.01 versus memory cells. P-values were calculated by a Student’s t test. Error bars show SEM.