Table II.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Genotype and/or relevant characteristics | Source |
|---|---|---|
| P. alvei CCM 2051T | Wild-type isolate; Kmr | Czech Collection of Microorganisms (CCM) |
| Escherichia coli DH5α | F−φ80dlacZ M15 (lacZYA-argF)U169 deoR recA1 endA1 hsdR17 (rK−mK−) phoA supE44 thi-1 gyrA96 relA1− | Invitrogen |
| Escherichia coli BL21 (DE) | F−, ompT, hsdS (rB−mB−), gal, dcm (DE3) | Invitrogen |
| P. alvei CCM 2051T ΔwsfP | P. alvei CCM 2051T carrying a targetron insertion at the wsfP locus | Zarschler et al. (2009) |
| P. alvei CCM 2051T ΔwsfPΔpglB | P. alvei CCM 2051T carrying a targetron insertions at the wsfP and the pglB locus | This study |
| P. alvei CCM 2051T ΔPAV_2c01630 | P. alvei CCM 2051T carrying a targetron insertion at the PAV_2c01630 locus | This study |
| P. alvei CCM 2051T ΔPAV_2c01640 | P. alvei CCM 2051T carrying a targetron insertion at the PAV_2c01640 locus | This study |
| P. alvei CCM 2051T ΔPAV_2c01630 comp | P. alvei CCM 2051T carrying a targetron insertion at the PAV_2c01630 locus and the pEXALV_P(1640)_1630 plasmid; Cmr | This study |
| P. alvei CCM 2051T ΔPAV_2c01640 comp | P. alvei CCM 2051T carrying a targetron insertion at the PAV_2c01640 locus and the pEXALV_P(1640)_1640 plasmid; Cmr | This study |
| P. alvei CCM 2051T (pEXALV_hag_6His) | P. alvei CCM 2051T wild-type isolate carrying the pEXALV_hag_6His plasmid; Cmr | This study |
| P. alvei CCM 2051T ΔPAV_2c01630 (pEXALV_hag_6His) | P. alvei CCM 2051T ΔPAV_2c01630 carrying the pEXALV_hag_6His plasmid; Cmr | This study |
| P. alvei CCM 2051T ΔPAV_2c01640 (pEXALV_hag_6His) | P. alvei CCM 2051T ΔPAV_2c01640 carrying the pEXALV_hag_6His plasmid; Cmr | This study |
| pEXALV | pNW33N carrying the sgsE S-layer gene promoter of G. stearothermophilus NRS 2004/3a; Cmr | Zarschler et al. (2009) |
| pET28a | Expression vector with a His6-tag, Kanr | Novagen |
| pTT_wsfA243 | pTT_wsfP1176 targeted for insertion at position 243/244 from the initial ATG of wsfA | Zarschler et al. (2010) |
| pTT_PAV2c_01630_1143 | pTT_wsfA243 targeted for insertion at position 1143/1144 from the initial ATG of PAV2c_01630 | This study |
| pTT_PAV2c_01640_273 | pTT_wsfA243 targeted for insertion at position 273/274 from the initial ATG of PAV2c_01640 | This study |
| pET28a_hag_6His | pET28a carrying the His6-tagged hag gene of P. alvei CCM 2051T; Kanr | This study |
| pEXALV_hag_6HIS | pEXALV carrying the his-tagged hag gene of P. alvei CCM 2051T | This study |
| pEXALV_P(1640)_1630 | pEXALV without P(SgsE) carrying the PAV_2c01630 gene and the promoter of PAV_2c01640 of P. alvei CCM 2051T | This study |
| pNW33N_P(1640)_1640 | pNW33N carrying the promoter and the PAV_2c01640 gene of P. alvei CCM 2051T | This study |