Table 1.

Radiochemical Yields of 6a via the prelabeling Pathway from 5a^a

	concentration of BocLys	6ab
		30 min	60 min
Organic conditionsc	10 mM	90%	94%
	50 mM	79%	85%
Aqueous conditionsd	10 mM	31%	46%
	50 mM	65%	68%

^aReactions were heated at 37 °C for the times indicated and then allowed to cool briefly before aliquots were removed for analysis by radio-HPLC using HPLC method 1.

^b

The radiochemical yields shown were calculated from radio-HPLC chromatograms and are corrected to account for the initial radiochemical purity of 5a from the crude reaction mixture prior to conjugation with BocLys according to the following equation:

$$\%\text{yield}=\frac{\%\text{radiochemical purity of BocLys conjugated complex}}{\%\text{radiochemical purity of initial isothiocyanate complex}}$$

^cOrganic conditions: mixed MeCN/phosphate buffer solutions with NEt₃ as the base.

^dAqueous conditions: aqueous pH 10.2 NaHCO₃ solutions.