FIG 6.
Convallatoxin does not inhibit IE2 gene transcription of virus-infected cells. MRC5 cells treated with DMSO, convallatoxin (5 nM or 50 nM), or flavopiridol (5 nM or 50 μM) were infected with AD169IE2-YFP and analyzed for mRNA transcripts of IE2 using qRT-PCR. Mock-infected cells were used as a control. Using the calculated CT values, the amount of IE2 transcripts relative to GAPDH transcripts was determined for each sample, and the average of the three replicates was plotted relative to DMSO. DMSO-treated cells were considered to have a relative IE2 transcript amount of 1, using standard errors (error bars).