FIG 6.

OBSL1 associates with the HPV16 entry platform. (A and B) HaCaT cells were exposed to HPV16 PsV for 4 h. The cells were fixed with methanol and treated with Click-iT reaction buffer, without the addition of Alexa Fluor label for vDNA staining. Obscurin-like 1 was stained with anti-OBSL1 antibody for staining of endogenous OBSL1 (red) (A) or with anti-V5 antibody after overexpression (B), L2 was stained with monoclonal mouse anti-L2 (L2-1) (green), and nuclear DNA was stained with Hoechst 33342 (blue). (C) HaCaT cells were cotransfected with OBSL1-V5 and CD151-green fluorescent protein (GFP) for 24 h and exposed to HPV16 PsVs for 4 h. OBSL1 was stained with monoclonal mouse V5 antibody (red), L1 was stained with polyclonal rabbit L1 antibody (K75; blue), and nuclei are shown with dotted lines.