FIG 7.

Expression of Wheat streak mosaic virus (WSMV) full-length and truncated coat protein (CP) in wheat infected by Triticum mosaic virus (TriMV) expressing WSMV CP or its deletion mutants. (A) Schematic diagram of the TriMV genome (a) with WSMV CP cistron (b) and CP cistron with a series of deletions (c to h) inserted between the P1 and HC-Pro cistrons. WSMV CP amino acids 101 to 300 were inserted into the TriMV genome (i). (B) Western blot assay of total proteins from wheat infected with TriMV or TriMV with WSMV CP or CP deletions at 14 days postinoculation. Below is an SDS-PAGE gel stained with Coomassie blue showing the amount of protein loaded per well for the Western blot assay. The blot was probed with WSMV polyclonal antibodies. Note that WSMV CP with deletion of aa 101 to 200 or 201 to 300 did not react with WSMV polyclonal antibodies while expression of WSMV CP aa 101 to 300 restored reactivity with WSMV antibodies. Letters a to i are as defined in panel A.