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. 2016 Nov 14;90(23):10886–10905. doi: 10.1128/JVI.01697-16

TABLE 3.

Mapping minimal regions of Wheat streak mosaic virus (WSMV) NIa-Pro and CP required for induction of superinfection exclusiona

Primary virus Challenge virus Expt 1
 Expt 2
Avg no. of local foci/leaf ± SE No. of plants systemically infected/no. of plants inoculatedb % of plants systemically infected with WSMV-GFP Avg no. of local foci/leaf ± SE No. of plants systemically infected/no. of plants inoculatedb % of plants systemically infected with WSMV-GFP
TriMV WSMV-GFP 32.0 ± 3.6 16/16 100 30.4 ± 2.8 19/19 100
TriMV-WSMV-NIa-Pro WSMV-GFP 0.2 ± 0.1 2/24* 8 1.1 ± 0.5 8/20* 40
TriMV-WSMV-NIa-ProΔ3-60aa WSMV-GFP 35.2 ± 4.3 15/15 100 14.8 ± 1.8 15/15 100
TriMV-WSMV-NIa-ProΔ61-120aa WSMV-GFP 40.4 ± 4.2 19/19 100 19.8 ± 2.6 18/18 100
TriMV-WSMV-NIa-ProΔ121-180aa WSMV-GFP 25.8 ± 2.9 18/18 100 14.9 ± 1.8 17/18 94
TriMV-WSMV-NIa-ProΔ181-229aa WSMV-GFP 42.5 ± 4.6 19/20 95 21.7 ± 2.7 19/19 100
WSMV WSMV-GFP 0.3 ± 0.2 0/17 0 0.0 ± 0.0 0/18 0
Buffer WSMV-GFP 62.4 ± 8.2 15/15 100 48.5 ± 4.6 18/18 100
TriMV WSMV-GFP 12.1 ± 1.0 16/16 100 79.9 ± 4.6 19/19 100
TriMV-WSMV-CP WSMV-GFP 0.3 ± 0.2 4/21* 19 1.7 ± 0.5 4/18* 22
TriMV-WSMV-CPΔ3-35aa WSMV-GFP 0.3 ± 0.2 2/20* 10 1.4 ± 0.5 7/19* 37
TriMV-WSMV-CPΔ36-84aa WSMV-GFP 0.1 ± 0.1 7/28* 25 5.1 ± 0.9 6/17* 35
TriMV-WSMV-CPΔ85-100aa WSMV-GFP 0.5 ± 0.2 2/18* 11 1.5 ± 0.5 1/16* 6
TriMV-WSMV-CPΔ101-200aa WSMV-GFP 15.6 ± 1.7 19/19 100 34.8 ± 3.9 16/16 100
TriMV-WSMV-CPΔ201-300aa WSMV-GFP 12.1 ± 0.9 19/19 100 40.5 ± 5.0 16/16 100
TriMV-WSMV-CPΔ294-349aa WSMV-GFP 0.5 ± 0.3 2/16* 13 1.6 ± 0.2 5/17* 29
TriMV-WSMV-CPaa101-300 WSMV-GFP 0.3 ± 0.2 4/15* 27 2.2 ± 0.4 7/17* 41
WSMV WSMV-GFP 0.0 ± 0.0 0/17 0 0.1 ± 0.1 0/18 0
Buffer WSMV-GFP 62.4 ± 8.2 15/15 100 118.9 ± 5.5 18/18 100
a

Wheat plants systemically infected with Triticum mosaic virus (TriMV) harboring WSMV cistrons were challenge inoculated with WSMV-GFP at 10 dpi. Local foci and systemic infection by the challenge virus (WSMV-GFP) on primary virus-infected wheat were examined at 5 and 9 days post-challenge inoculation for local fluorescent foci and the number of systemically infected plants, respectively. Presented local foci per leaf are average numbers of foci from 15 to 20 inoculated leaves.

b

Asterisks indicate that 1 to 10 isolated infection foci per leaf were observed in upper noninoculated leaves.