TABLE 4.

Examining Triticum mosaic virus (TriMV)-encoded NIa-VPg, NIa-Pro, and CP cistrons for superinfection exclusion activity in wheat^a

Primary virus	Challenge virus	Expt 1			Expt 2
		Avg no. of local foci/leaf ± SE	No. of plants systemically infected/no. of plants inoculatedb	% of plants systemically infected with TriMV-GFP	Avg no. of local foci/leaf ± SE	No. of plants systemically infected/no. of plants inoculated	% of plants systemically infected with TriMV-GFP
WSMV	TriMV-GFP	18.6 ± 1.8	18/18	100	12.6 ± 0.8	17/17	100
WSMV-TriMV-NIa-VPg	TriMV-GFP	16.6 ± 1.3	22/22	100	19.2 ± 2.7	20/20	100
WSMV-TriMV-NIa-Pro	TriMV-GFP	0.8 ± 0.2	20/20	100	0.8 ± 0.3	19/19	100
WSMV-TriMV-CP	TriMV-GFP	0.1 ± 0.1	1/22*	5	0.0 ± 0.0	1/19	5
TriMV	TriMV-GFP	0.1 ± 0.1	0/18	0	0.0 ± 0.0	0/14	0
Buffer	TriMV-GFP	89.8 ± 7.0	16/16	100	79.3 ± 6.7	15/15	100

^aWheat plants systemically infected with Wheat streak mosaic virus (WSMV) harboring TriMV NIa-VPg, NIa-Pro, or CP were challenge inoculated with TriMV-GFP. Formation of local foci and systemic infection by the challenge virus were observed at 5 and 12 days post-challenge inoculation.

^bThe asterisk indicates that 1 to 10 isolated infection foci per leaf were observed in upper noninoculated leaves.