FIG 5.

Dependence on CD81 and CLDN1 for growth-adapted mutants is similar to that of the parental virus. Huh7.5 cells were preincubated with a dilution series of anti-CD81 (A) or anti-CLDN1 (B) for 1 h at 37°C and then infected with parental and growth-adapted mutant viruses. Infection was quantified 72 h later by FFA and EC₅₀ determined by nonlinear regression. For each mutant virus, the fold change in EC₅₀ for the antibody treatment relative to parental virus is plotted. Asterisks indicate differences that are statistically significant (*, P < 0.05; **, P < 0.01; ns, not significant). Error bars represent SEM. The results are averages from three independent experiments performed in triplicate or quadruplicate.