FIG 2.

Stabilization of TRAF6 in HCV-infected cells by bafilomycin A1 but not by MG132. Huh7 cells were infected with HCV for 24 h and then treated with 5 or 10 μM MG132 dissolved in dimethyl sulfoxide (DMSO) for 16 h (A) or with 100 nM bafilomycin A1 (BafiloA1) for 24 h (B). Cells were then lysed for immunoblot analysis. (C) Huh7 cells were treated with rapamycin (10 μM) for the indicated lengths of time and lysed for immunoblot analysis. M, mock-treated cells. (D) Huh7 cells and HCV subgenomic RNA replicon cells were lysed for immunoblot analysis.