FIG 4.

Analysis of the interaction between p62 and TRAF6 in HCV-infected cells. (A) Huh7 cells were transfected with the control siRNA (siControl) or the p62 siRNA (sip62) twice on consecutive days and then infected with HCV for 24 or 48 h using an MOI of 1. The cells were then lysed for immunoblot analysis. (B) Huh7 cells were infected with HCV for 24 or 48 h. The cells were then lysed and immunoprecipitated (IP) with either the control IgG or the anti-p62 antibody followed by immunoblot analysis (IB) using the anti-TRAF6 and anti-p62 antibodies (top two blots). The total cell lysates were also used for immunoblotting to serve as the input control.