FIG 1.

Schematic designs for UL31 and UL34 showing relevant sequence features. (A) Protein sequence of pUL34 showing the location of the Q163A mutation, conserved regions, and the pUL31 interaction region. (B) Protein sequence of pUL31 showing the location of the R229L mutation, conserved regions, and the pUL34 interaction region. (C) Three-dimensional structures of pUL31 and pUL34. The locations of the Q163A mutation and the R229L mutation are indicated with arrows. Designations of conserved domains of pUL31 and pUL34 follow the nomenclature proposed by Schnee et al. (22). The protein structure of HSV-1 NEC is that previously described by Bigalke and Heldwein (31).