FIG 11.

UL31(R229L) does not regain the ability to disrupt the nuclear lamina. (A to L) Representative confocal images of lamin A/C in uninfected and infected cells. Vero cells (top row), UL34WT-expressing cells (middle row), and Q163A-expressing cells (bottom row) were either left uninfected (A to C) or infected with HSV-1(F) (D to F), the UL34-null virus (G to I), or the UL34-null/UL31(R229L) virus (J to L). Cells were fixed at 18 hpi and stained for lamin A/C. (M) Nuclear contour ratios for UL34-null/UL31(R229L)-infected cells. One hundred nuclei were used to calculate the nuclear contour ratio for each condition. Statistical significance was determined by using Student's t test.