UL34(Q163A) causes an inability to disrupt the nuclear lamina. (A to U) Representative confocal images of UL34 and lamin A/C in infected and uninfected cells. (A to C) Uninfected Vero cells, UL34WT-expressing cells, and Q163A-expressing cells were fixed and stained for lamin A/C. Vero cells (D to I), UL34WT-expressing cells (J to O), or Q163A-expressing cells (P to U) were infected with HSV-1(F) (D to F, J to L, and P to R) or the UL34-null virus (G to I, M to O, and S to U). Cells were fixed at 16 hpi and stained for pUL34 or lamin A/C. (V and W) Nuclear contour ratios for cells infected with HSV-1(F) (V) or the UL34-null virus (W).