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. 2016 Nov 14;90(23):10738–10751. doi: 10.1128/JVI.01544-16

FIG 9.

FIG 9

Emerin localization. (A to AA) Confocal images of pUL34 (left column) and emerin (center column) in infected and uninfected cells. Vero (A to I), UL34WT-expressing (J to R), and Q163A-expressing (S to AA) cells were infected with no virus (A to C, J to L, and S to U), HSV-1(F) (D to F, M to O, and V to X), or the UL34-null virus (G to I, P to R, and Y to AA). Cells were fixed at 16 hpi and stained for pUL34 or emerin. (AB) Western blot of Vero (lanes 1 to 3), UL34WT-expressing (lanes 4 to 7), and Q163A-expressing (lanes 7 to 9) cells. Cells were left uninfected (lanes 1, 4, and 7) or infected with HSV-1(F) (lanes 2, 5, and 8) or the UL34-null virus (lanes 3, 6, and 9). Cell lysates were collected at 15 hpi, and Western blotting was performed as described in Materials and Methods, with probing for emerin.