FIG 2.

Genome stability of selected rIHNVs after 2 and 10 passages (P2 and P10) in cell culture. rIHNVs were passaged up to 10 times on EPC cells. At the second and tenth passages, viral RNA was extracted from infected cell supernatants to confirm the gene order of each rIHNV. Agarose gel electrophoresis of RT-PCR products amplified from the rIHNV genome was performed using specific N and G primers.