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ELISPOT analysis of IgM-secreting cells in head kidney leukocytes following N2G3 or mock immunization. Thirty days postimmunization, fish were sacrificed and the head kidney was removed. ELISPOT assay was performed on isolated kidney leukocytes. Values are expressed as IgM-secreting cells in 105 leukocytes from individual fish tested. Bars indicate mean values in each group, and the asterisk denotes significant differences between groups (P < 0.05).
