TABLE 3.
Sensitivities of VIR165-resistant and -dependent mutants to a series of antibodies and entry and fusion inhibitorsc
| Virus | HIVIg |
sCD4 |
CD4-IgG2 |
AMD3100 |
T20 |
|||||
|---|---|---|---|---|---|---|---|---|---|---|
| IC50 (μg/ml) | Fold change in resistanceb | IC50 (μg/ml) | Fold change in resistanceb | IC50 (μg/ml) | Fold change in resistanceb | IC50 (μg/ml) | Fold change in resistanceb | IC50 (ng/ml) | Fold change in resistanceb | |
| WT | 2.40 | 1.0 | 2.30 | 1.0 | 2.29 | 1.0 | 1.85 | 1.0 | 54.9 | 1.0 |
| Mutant viruses | ||||||||||
| V42I | 4.90 | 2.0 | 2.57 | 1.1 | 6.98 | 3.1 | 2.02 | 1.1 | 68.9 | 1.3 |
| A58V | 2.58 | 1.1 | 3.01 | 1.3 | 2.14 | 0.9 | 1.96 | 1.1 | 14.7 | 0.3 |
| D62N | 3.08 | 1.3 | 2.10 | 0.9 | 3.76 | 1.6 | 2.08 | 1.1 | 53.3 | 1.0 |
| E64Ka | 2.76 | 1.2 | 9.84 | 4.3 | 7.88 | 3.4 | 0.29 | 0.2 | 22.0 | 0.4 |
| H66Ra | 1.84 | 0.8 | 30.30 | 13.2 | 14.42 | 6.3 | 0.58 | 0.3 | 16.5 | 0.3 |
| A558T | 2.40 | 1.0 | 0.99 | 0.4 | 2.07 | 0.9 | 1.26 | 0.7 | 6.7 | 0.1 |
| A549A | ND | ND | ND | ND | 2,983 | 54.3 | ||||
| A549A/N637K | ND | ND | ND | ND | 1,426 | 26.0 | ||||
| Q577R | ND | ND | ND | ND | 71.4 | 1.3 | ||||
a
Because VIR165-dependent mutants are not infectious in the absence of VIR165, 10 μg/ml VIR165 was present for the A60E, E64K, and H66R mutants.
b
Compared to that of the WT. Values in bold represent ≥3-fold more resistance than that of the WT; values in bold italics represent ≥3-fold more sensitivity than that of the WT.
c
ND, not done.