TABLE 4.
Stability of VIR165-resistant and -dependent mutants in the absence or presence of sCD4a
| Virus | Decay at 37°C |
Thermostability |
||||
|---|---|---|---|---|---|---|
| t1/2 (h) | t1/2 (+sCD4) (h) | Δt1/2 (h) | Tm (°C) | Tm (+sCD4) (°C) | ΔTm (+sCD4) (°C) | |
| WT | 11.3 (0.9) | 6.1 (0.3) | −5.2 (1.0) | 44.6 (0.3) | 41.9 (0.2) | 2.7 (0.3) |
| Mutant viruses | ||||||
| A58V | 8.2 (1.1) | 5.6 (0.6) | −2.6 (0.5) | 44.8 (0.2) | 42.1 (0.1) | 2.7 (0.2) |
| A60E | 9.4 (0.5) | 7.0 (0.4) | −2.4 (0.6) | 44.7 (0.2) | 43.3 (0.1) | 1.4 (0.1) |
| E64K | 9.5 (1.4) | 8.0 (1.2) | −1.5 (0.9) | 44.6 (0.2) | 43.6 (0.1) | 0.9 (0.1) |
| H66R | 8.2 (1.3) | 7.9 (1.1) | −0.3 (0.5) | 44.7 (0.2) | 44.2 (0.4) | 0.5 (0.3) |
a
Average values are shown for at least three independent experiments, each performed in duplicate, with standard errors of the means (SEM) shown in parentheses.