FIG 7.

Total and species-specific IgG1 antibody titers to RV-A and RV-C VP1 antigens for the 20 donors. The geometric means and 95% confidence intervals are indicated. A titration of reference sera was included for the quantification of IgG1 binding, and a negative control was used to determine nonspecific binding. Species-specific measurements were conducted after preabsorption of plasma in a lysate mixture of E. coli producing the other two RV species. The limit of detection was 250 ng/ml. A P value of <0.05 (indicated by the asterisks) was considered significant. The analyses of total versus species-specific measurements were done using a paired Wilcoxon test. Analyses were performed using GraphPad (La Jolla, CA, USA) Prism software.