TABLE 1.
Primers used in this study
| Primer function and namea | Sequence (5′→3′) |
|---|---|
| Eukaryotic expression | |
| pcDNA3.1-P-F | CGCGGATCCATGTCTCTACATTCGAAATTG |
| pcDNA3.1-P-R | CCGCTCGAGTTACAACCTATATTTTTGATAC |
| pCMV-Myc-P-F | CCGGAATTCCGATGTCTCTACATTCGAAATTG |
| pCMV-Myc-P-R | CCGCTCGAGTTACAACCTATATTTTTGATAC |
| pCMV-HA/Myc-TBK1-F | CCGGAATTCCGATGCAGAGTACGGCCAAT |
| pCMV-Tag2c-TBK1-F | CGCGGATCCCGATGCAGAGTACGGCCAAT |
| pCMV-HA/Myc/Tag2C-TBK1-R | AACTCGAGTCACATCCGCTCCACTG |
| pCMV-HA/Myc/Tag2C-TBK1-ΔN-F | CCGGAATTCCGATGCAGAGTACGGCCAATTACCTGTGGATGATGTCCGACCTGACAGCGAACCTCTTC |
| pCMV-HA/Myc/Tag2C-TBK1-ΔN-R | AACTCGAGTCACATCCGCTCCACTG |
| pCMV-HA/Myc/Tag2C-TBK1-N-F | CCGGAATTCCGCTGGGTCAGGGAGCCACAGC |
| pCMV-HA/Myc/Tag2C-TBK1-N-R | AACTCGAGTTAGTTGTAAGTGTGTATGTAG |
| pCMV-HA/Myc/Tag2C-MITA-F | CCGGAATTCCGATGTCTGTGATGGGAGAA |
| pCMV-HA/Myc/Tag2C-MITA-R | CCGCTCGAG TTAGTTTTGTTTCATTGC |
| pCMV-HA/Myc/Tag2C-IRF3-F | CCGGAATTCCGATGACTCAAGCAAAACCG |
| pCMV-HA/Myc/Tag2C-IRF3-R | AACTCGAGTTAGCAGAGCTCCATCA |
| pCMV-HA/Myc/Tag2C-IRF7-F | CCGGAATTCCGATGCAGAGCACAAATGC |
| pCMV-HA/Myc/Tag2C-IRF7-R | AACTCGAGTTATTCCACTGAAGGCA |
| pEGFP-N3-P-F | CTAGCTAGCATGTCTCTACATTCGAAATTG |
| pEGFP-N3-P-R | CGCGGATCCCAACCTATATTTTTGATAC |
| pDsRed-TBK1-F | CGCGGATCCGCCACCATGCAGAGTACGGCCAAT |
| pDsRed-TBK1-R | CCGGAATTCCGCATCCGCTCCACTG |
| Real-time PCR | |
| IFN-EPC-F | ATGAAAACTCAAATGTGGACGTA |
| IFN-EPC-R | GATAGTTTCCACCCATTTCCTTAA |
| RIG-I-EPC-F | TGCTGGACCGGATGTGTTATCT |
| RIG-I-EPC-R | TGGTGATCGATGGTTCGATTCT |
| ISG15-1-EPC-F | CAGCCTTGAGGATGATTCCAG |
| ISG15-1-EPC-R | TGCCGTTGTAAATCAGTCG |
| ISG15-2-EPC-F | GCCTGGTATCACAGACAG |
| ISG15-2-EPC-R | ACATCTTGCACTGACATA |
| MAVS-EPC-F | GAATGTCCCTGTCCGAGAAA |
| MAVS-EPC-R | TCTGAACATGCTCGTTTGCAG |
| qP-F | TTGGACCTGGGATAGTGA |
| qP-R | CTTGCTTGGTTTGTGGG |
| qG-F | CGACCTGGATTAGACTTG |
| qG-R | AATGTTCCGTTTCTCACT |
| qM-F | TACTCCTCCCACTTACGA |
| qM-R | CAAGAGTCCGAGAAGGTC |
| qβ-actin-F | CACTGTGCCCATCTACGAG |
| qβ-actin-R | CCATCTCCTGCTCGAAGTC |
a
F, forward; R, reverse.