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. Author manuscript; available in PMC: 2017 Sep 20.
Published in final edited form as: Environ Sci Technol. 2016 Sep 9;50(18):10226–10235. doi: 10.1021/acs.est.6b02108

Figure 4.

Figure 4

[3H]TCDD binding by in vitro expressed AHRs from chicken, common tern, and roseate tern. (A) In vitro transcription and translation of AHRs. AHRs were expressed in the presence of [35S]methionine. (B, C) AHRs were incubated overnight at 4°C with [3H]TCDD (B, 2 nM; C, 8 nM final concentration) and then analyzed by velocity sedimentation. Binding is measured in disintegrations per minute (dpm), where binding of [3H]TCDD to unprogrammed lysate (UPL, i.e., without AHR) measures nonspecific binding, and specific binding = total binding (radioligand binding to AHR) - nonspecific binding (radioligand binding to UPL).