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. Author manuscript; available in PMC: 2017 Jan 21.
Published in final edited form as: Mol Cell. 2015 Dec 17;61(2):222–235. doi: 10.1016/j.molcel.2015.11.015

Figure 3. sidRNAs Are Associated with AGO4.

Figure 3

(A) Heatmap showing the size distribution of AGO4-associated sRNAs from sidRNA-producing loci in Col-0, dcl2/3/4 and dcl1/2/3/4. Color intensity represents the fraction of sRNAs of different sizes.

(B) Box plots of the percentages of laddered AGO4-associated sRNAs produced from sidRNA loci in Col-0 and dcl2/3/4.

(C) A representative group of laddered AGO4-associated sRNAs produced from AtREP2. Numbers represent the abundance (RPM) of sidRNAs with different lengths ranging from 19 to 29 nt. Values in the brackets indicate the percentages.

(D) Detection of sRNAs at representative sidRNA loci in total extracts and AGO4 immunoprecipitates by Northern blot. 5S rRNAs stained with ethidium bromide were used as loading controls for total extracts. A silver-stained gel shows that comparable amounts of AGO4 immunoprecipitates were used for sRNA extraction.