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. Author manuscript; available in PMC: 2017 Oct 1.
Published in final edited form as: Radiother Oncol. 2016 Sep 22;121(1):162–168. doi: 10.1016/j.radonc.2016.08.029

Fig. 2.

Fig. 2

PU-H71 inhibits DSB repair in carbon irradiated cancer cells. HeLa-SQ5 (A), H1299 (B), and A549 (C) cells were fixed at various times (1, 6 or 24 h) after 2 Gy carbon ion irradiation. The cells were stained using anti-γ-H2AX (Ser139) antibodies with DAPI as nuclear counter stain. The number of γ-H2AX foci was counted in >50 nuclei per experiment. All the data in this figure were obtained by subtracting the number of γ-H2AX foci in non-irradiated control values (PU-H71 treated or mock treated group). Data represent mean ± SEM for two or three determinations. * indicates p < 0.05.