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. 2015 Dec 30;56(13):8258–8267. doi: 10.1167/iovs.15-17073

Figure 1.

Figure 1

Reprogramming Tie2 GFP fibroblasts into iPSCs. (A) Green fluorescence protein expression in Tie2-GFP heart and lung primary ECs. (B) Tail-tip fibroblasts were isolated from Tie2-GFP mice and reprogrammed into (C) iPSCs via transduction with either (D) fluorescently tagged lentiviral vectors (iPSC-L1) or a (J) polycistronic lentiviral vector (iPSC-L2). (E) The FACS iPSC-L1 cells did not express the exogenous fluorescent tags eGFP-Oct4 and mKate-c-Myc (4′,6-diamidino-2-phenylendole [DAPI] = blue), but did express (F) Nanog, indicating the iPSC-L1 cells were fully reprogrammed. (G) iPSC-L1 EBs expressed GFP (green), indicating the presence of ECs (DAPI = blue). (H, K) Reverse transcription-PCR analysis of pluripotency markers in both iPSC lines (iPSC-L1 and iPSC-L2, respectively). (J) Nanog expression (green) in iPSC-L2 iPSCs (DAPI = blue). (I, L) Teratoma formation assays for iPSC-L1 and iPSC-L2, respectively. ∧ = endoderm, * = ectoderm, + = mesoderm). Scale bars: 100 μm.