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. 2015 Dec 30;56(13):8258–8267. doi: 10.1167/iovs.15-17073

Figure 2.

Figure 2

Spontaneous iPSC-EC differentiation, kidney coculture iPSC-EC differentiation, and previously published iPSC- EC differentiation protocol. (A–D) Spontaneously differentiated iPSC-derived ECs express EC markers (A) VE-Cadherin and (B) ZO-1, a small amount of (C) TTR, but no (D) CA4. (E–H) iPSC-derived ECs differentiated in coculture with primary mouse kidney ECs also express (E) VE-Cadherin, (F) ZO-1, and (G) TTR, but not (H) CA4. (I–L) Likewise, iPSC-derived ECs differentiated using a previously published iPSC-EC differentiation protocol from Rufaihah et al.18 also express (I) VE-Cadherin, (J) ZO-1, and (K) TTR, but not (L) CA4. DAPI = blue. Scale bars: 100 μm.