Figure 3. Analysis of tg(vsx2.2:GFP-caax) clones show uncoupled oscillations at apical and basal surfaces.

(A) Scheme of transplantation experiment at sphere stage. (B) Confocal microscopy image showing transmitted light and GFP expression for transplanted clones (white arrows) at 20 hpf. Antero-posterior (a–p) axis is indicated. (C–E) Confocal microscopy time-lapse images show length variation of basal (orange) and apical (purple) edges through time in a transplanted clone. The orientation of the apico-basal (a–b) axis is indicated. Scale bars = 50 µm in B and 10 µm in C–E. (F) Quantification of the basal (orange) and apical (purple) length variation for an individual clone showing no correlation between the oscillations (R = 0064). (G) Box plot showing the distribution of apical vs basal oscillations correlation coefficients for 10 transplanted neuroblasts from five different retinas.

DOI: http://dx.doi.org/10.7554/eLife.15797.010

Figure 3—figure supplement 1. Mitotic rounding impact on basal constriction and apical expansion.

(A–D) Confocal microscopy time-lapse images showing a mitosis in a tg(vsx2.2:GFP-caax) retina at 20 hpf. Dashed white lines highlight flanking cells. Arrows indicate apical (purple) and basal (orange) distance variation. The orientation of the apico-basal (a–b) axis is indicated. (E–F) The graphs show the quantification of distance variation (%) for the apical (E) and basal (F) sides. The mitotic event (red arrow) results only in a transient expansion of the apical domain. Error bars indicate standard error of the mean (n = 10, from three different retinas). (G–I) Confocal microscopy time-lapse images showing a mitosis occurring in the apical plane in a tg(vsx2.2:GFP-caax) retina at 20 hpf. Dashed white arrows indicate apical distance variation along the mitotic axis. Neighboring cells are indicated with colored dots. (J) Quantification of apical distance variation (%) along the mitotic axis for five different cells confirms a transient expansion of the apical domain. Scale bars = 10 µm.

DOI: http://dx.doi.org/10.7554/eLife.15797.011