Video 4. Actin dynamics in constricting retinal cells.
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DOI: 10.7554/eLife.15797.014
(Upper panel) Maximum projection of 3 z-stacks (over a total of 1 µm) along the apico-basal axis shows actin oscillatory activity in tg(vsx2.2:lyn-tdTomato) embryos at 20 hpf. Retinal basal surface (region within the square) is magnified in lower panels. (Lower panels) Time lapse shows the simultaneous recording of membrane behavior, as revealed by lyn-tdTomato (left panel), and actin dynamics, as revealed by Utrophin-GFP (right panel). Images were acquired every 5 s. Scale bars = 10 µm. See also Figure 4.