Fig. (2).

Analysis of the cytotoxic properties of various 1,5-diaryl-3-oxo-1,4-pentadienes 1–10 against hematological malignancies. The cytotoxic potential of 10 curcumin analogues was studied in 13 cell lines by DNS. The compounds were tested at a final concentration of 1 μM and incubated for 20 h. For ease of presentation, a heat map was used to depict the results that shows a gradient from green (no toxicity) to red (100% toxicity) with black representing ~50% cytotoxicity. The most affected cell lines (red squares) were Ramos, Nalm-6, Jurkat, and CEM. The two non-transformed cell lines tested, MCF-10A and Hs-27, were not significantly affected by the test compound after 20 h of exposure. Note that 1 at the top of the heat map was selected for further characterization as it exhibited cytotoxic activity against the majority of leukemia/lymphoma cell lines but not the non-transformed cell lines. The MeV heat-map generating software was used to construct the heat maps.