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. 2016 Apr 19;57(4):1939–1950. doi: 10.1167/iovs.15-18789

Figure 6.

Figure 6

MYOC secretion from NTM-5 cells parallels secretion from HEK-293T cells (A) GLuc luminescence assay of secreted MYOC eGLuc2 variants (same color designation as described in Fig. 6). Conditioned media aliquots (50 μL) from NTM-5 cells 48 hours post-transfection were assayed for the MYOC eGLuc2 fusion protein (n = 3 independent experiments, ±SD, *P < 0.05, **P < 0.01, ***P < 0.001 versus hypothetical mean value of 100). (B) Temperature-sensitive secretion of MYOC variants in NTM-5 cells. NTM-5 cells were transfected for 48 hours, followed by a media change and grown at 37°C or 30°C for up to 48 hours. At 24 and 48 hours after temperature shift media aliquots were taken and assayed for eGLuc2. Results are presented as a percentage of the 37°C samples' secretion (n = 3 independent experiments, ±SD, *P < 0.05 versus WT MYOC). (C) Temperature-sensitive secretion of MYOC between HEK-293T and NTM-5 cells 24 hours after temperature shift. An average of three independent temperature-shift experiments (performed 24 hours post growth temperature reduction) in HEK-293T and NTM-5 cells were plotted against each other.