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. Author manuscript; available in PMC: 2017 Dec 1.
Published in final edited form as: Virology. 2016 Oct 13;499:350–360. doi: 10.1016/j.virol.2016.09.025

Figure 1.

Figure 1

C15 adaptation. (A) Monolayers of HeLa-E8 and HeLa-H1, or differentiated cultures of PBE-ALI cells from three donors, grown in 12-well plates were infected with A16, A16/pR16.11, C15 or C15a virus at 4 × 108 vRNA copies per well. After 2 h (input virus to estimate binding) or 24 h (replication) incubation at 34°C, the cells were analyzed for viral RNA signals by RT-qPCR (means ± s.d., n ≥ 3). (B) C15 virus was passaged serially in HeLa-E8 cells as described in Results and each passage was evaluated 72 h p.i. for virus load (viral RNA copies per ml) by RT-qPCR (means ± s.d., n = 3). Polyclonal C15a is defined as the Passage 10 material. (C) Passage 1, Passage 5 and Passage 10 and mock-infected cells were visualized (72 h p.i.) and photographed by light microscopy. Scale bar is 100 μm.