Skip to main content
. Author manuscript; available in PMC: 2017 Dec 1.
Published in final edited form as: Virology. 2016 Oct 13;499:350–360. doi: 10.1016/j.virol.2016.09.025

Figure 3.

Figure 3

C15a recombinants. (A) Recombinant C15 and recombinant C15 harboring one or both dominant adaptive mutations (VP1 T125K, 3A T41K) and polyclonal C15a were tested for relative binding affinities (cell-associated input virus at 2 h) and replication potential (24 h) in HeLa-E8 and HeLa-H1 cells. Infections per well were initiated with equivalent 4 × 108 vRNA copies per well. Viral load was measured by RT-qPCR (means ± s.d., n = 5). (*) indicates p < 0.05 significance (t-test) vs. C15. (B) Infected cells were visualized and photographed by light microscopy at 48 h p.i. Scale bar is 100 μm. (C) Cellular cytotoxicity (LDH release) caused by recombinant and polyclonal C15a viruses in HeLa-E8 cells over the time-course of infection (OD490, means ± s.d., n = 3). (*) indicates p < 0.05 significance (t-test) vs. C15.