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Expression analysis of inflammatory cytokine and chemokine genes using real-time polymerase chain reaction (PCR). RNA from uninjured untreated control disks and injured disks treated with neonatal human dermal fibroblasts (nHDFs) or saline was isolated for real-time PCR analysis. At 2 weeks after treatment, expression levels of interleukin-8 (IL8) and chemokine (C-C motif) ligand 2 (CCL2) genes were analyzed. Error bars represent the standard error of the mean.
