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. 2004 Jul 26;101(32):11668–11672. doi: 10.1073/pnas.0403499101

Fig. 2.

Fig. 2.

Analysis of hydrophobic extracts of eye cups excised from Abcr+/+ mice (age 7 months) with the Leu-450 or Met-450 variant of Rpe65. (A and B) Typical chromatograms obtained by reverse-phase HPLC with monitoring at 430 nm illustrate the detection of A2E and iso-A2E. (C) Quantitation of A2E and iso-A2E in eye cups of Abcr+/+ mice with either the Leu-450 or Met-450 polymorphism. Levels were determined as integrated peak areas normalized to an external standard of A2E. Values are expressed as picomoles per eye and for each group are based on measures obtained after pooling eight eyes into a single sample.