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. Author manuscript; available in PMC: 2017 Apr 17.
Published in final edited form as: Nat Chem Biol. 2016 Oct 17;12(12):1075–1083. doi: 10.1038/nchembio.2204

Figure 5. Hippocampal ligands of PPARα induce PPRE-driven luciferase activity in primary mouse astrocytes and neurons.

Figure 5

Astrocytes plated at 60–70% confluence were transfected with tk-PPREx3-Luc, a PPRE-dependent luciferase reporter construct. After 24 h of transfection, cells were treated with different concentrations of HEX (A), OCT (B) and HMB (C) for 4 h followed by monitoring luciferase activity. Results are mean ± SD of three independent experiments. ap< 0.001 vs. control. Ppara-null astrocytes were transduced with lentivirions containing empty vector (D), FL-Ppara (E), Y314D-Ppara (F), Y464D-Ppara (G), and Y314D/Y464D-Ppara (H) for 48 h followed by transfection with tk-PPREx3-Luc. After 24 h of transfection, cells were treated with different doses of HEX, OCT and HMB for 4 h followed by monitoring luciferase activity. PPRE luciferase activity was assayed in Ppara-null astrocytes transduced with lentivirions containing empty vector (I), FL-Ppara (J), Y314D-Ppara (K), Y464D-Ppara (L), and Y314D/Y464D-Ppara (M) after treatment with different doses of WY14643, fenofibrate, and clofibrate. Results are mean ± SD of three independent experiments. ap< 0.001 vs. control.