Skip to main content
. Author manuscript; available in PMC: 2018 Jan 1.
Published in final edited form as: Exp Neurol. 2016 Aug 12;287(Pt 1):54–64. doi: 10.1016/j.expneurol.2016.08.006

Figure 3.

Figure 3

Ketogenic Diet (KD) treatment increases nuclear content of PPARγ2 in brain tissue. Wild-type (WT) and Kv1.1KO mice were weaned onto both a standard diet (SD) or KD for 10-4 days and the drinking water contained either vehicle or the PPARγ antagonist GW9662. The mice were euthanized and brains were processed for western blot analysis. (a) Example western blots of the genotypes and treatment groups. (b) PPARγ1 and γ2 bands normalized to β-actin. (b1) PPARγ2/β-actin ratio (n = 4–10 mice; two-way ANOVA, interaction: F(3,40) = 0.224, P = 0.8792, treatment: F(3,40) = 8.555, P = 0.0002, genotype: F(1,40) = 2.627, P = 0.1129; Tukey’s multiple comparisons post-hoc test, *P < 0.05, **P < 0.001 as compared to mice fed an SD within genotype). (b2) PPARγ1/β-actin ratio (n = 4–10 mice; two-way ANOVA, interaction: F(3,40) = 0.9445, P = 0.4282, treatment: F(3,40) = 0.3968, P = 0.756, genotype: F(1,40) = 18.03, P = 0.0001; Tukey’s multiple comparisons post-hoc test, *P < 0.05 between mice fed a KD in each genotype). (b3) PPARγ2/ PPARγ1 ratio (n = 4–10 mice; two-way ANOVA, interaction: F(3,40) = 3.434, P = 0.0258, treatment: F(3,40) = 6.14, P = 0.0016, genotype: F(1,40) = 15.96, P = 0.0003; Tukey’s multiple comparisons post-hoc test, **P < 0.05, ***P < 0.001 as compared to mice fed an SD within genotype, ###P < 0.001 between mice fed a KD in each genotype). (c) PPARγ/β-actin ratios normalized to wild-type mice fed a SD. (c1) PPARγ2 (n = 4–10 mice; two-way ANOVA, interaction: F(3,40) = 0.7943, P = 0.5043, treatment: F(3,40) = 8.976, P = 0.0001, genotype: F(1,40) = 2.619, P = 0.1135; Tukey’s multiple comparisons post-hoc test, *P < 0.05, **P < 0.01 as compared to mice fed an SD within genotype). (c2) PPARγ1 (n = 4–10 mice; two-way ANOVA, interaction: F(3,40) = 1.582, P = 0.2088, treatment: F(3,40) = 0.3805, P = 0.7676, genotype: F(1,40) = 14.55, P = 0.0005; Tukey’s multiple comparisons post-hoc test, *P < 0.05 between mice fed a KD in each genotype). (c3) PPARγ2/ PPARγ1 ratio (n = 4–10 mice; two-way ANOVA, interaction: F(3,40) = 3.003, P = 0.0416, treatment: F(3,40) = 5.278, P = 0.0037, genotype: F(1,40) = 15.05, P = 0.0004; Tukey’s multiple comparisons post-hoc test, **P < 0.01, ***P < 0.001 as compared to mice fed an SD within genotype, ###P < 0.001 between mice fed a KD in each genotype). Numbers in each bar graph indicate the number of animals in each group.