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. Author manuscript; available in PMC: 2017 Dec 1.
Published in final edited form as: Anesthesiology. 2016 Dec;125(6):1159–1170. doi: 10.1097/ALN.0000000000001358

Figure 3.

Figure 3

Anesthesia-induced apoptotic cell death in two week-old dentate granule cells (DGC) does not lead to long-term diminution in neuronal density in this population. Confocal maximum projections show immunostaining for green fluorescent protein (GFP) in representative two month-old animals exposed to six hours of 1.5% isoflurane (anesthesia) or room air (control) on P21. No differences between control and anesthesia-treated mice were found. Scale bar = 250 μm. Scatterplot shows the density of GFP-expressing cells in the dentate granule cell body layer. Circles represent individual animal means, while squares reflect group means ± SEM. The two groups were statistically equivalent (P=0.915, Student's t-test).