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. Author manuscript; available in PMC: 2017 Dec 1.
Published in final edited form as: Anesthesiology. 2016 Dec;125(6):1159–1170. doi: 10.1097/ALN.0000000000001358

Figure 4.

Figure 4

Anesthesia-induced apoptotic cell death in two week-old dentate granule cells does not lead to alterations in neuronal proliferation in this population. Confocal maximum projections showing green fluorescent protein (GFP)+Ki67 (A) and GFP+Calretinin (B) immunostaining in the hippocampal granule cell layer. Double-labeled cells are denoted by arrows. Scale bar = 30 μm. Scatterplots show the percentage of GFP-expressing cells double-labeled with Ki67 (C) or calretinin (D). Circles represent individual animal means, while squares reflect group means ± SEM. The two groups were statistically equivalent for both Ki67 and calretinin.