Fig. 2.

Increased AGE-R1 expression blocks AGE-induced NF-κB activation in R1-MC. (A) Dose–response. (B) Time course. NF-κB activity was measured by using a luciferase expression plasmid and after incubation with different concentrations of AGE-BSA for different intervals. Luciferase expression in unstimulated cells served as control and as baseline for NF-κB activity (100%). Each experiment was performed in triplicate, and experiments were repeated at least three times. Data are means ± SD. *, P < 0.01 vs. unstimulated control; #, P < 0.01 vs. mock-MC.