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Kinetic parameters of EstDZ3-mediated hydrolysis against various pNP-esters.
| Substrate (pNP ester) | Km (mM) | Vmax (μmol⋅min-1⋅mg-1) | kcat (s-1) | kcat/Km (s-1⋅mM-1) |
|---|---|---|---|---|
| Acetate (C2) | 0.30 ± 0.07 | 355.3 ± 32.9 | 740 | 2,428 |
| Butyrate (C4) | 0.15 ± 0.02 | 906.4 ± 46.0 | 1,888 | 12,464 |
| Caprylate (C8) | 0.19 ± 0.01 | 500.6 ± 11.6 | 104 | 557 |
| Caprate (C10) | 0.17 ± 0.01 | 386.7 ± 8.2 | 80 | 471 |
| Laurate (C12) | 0.61 ± 0.14 | 357.1 ± 45.1 | 743 | 1,268 |
